Slender structural floors could experience irritating vibration problems due to human walking load and so, vibration acceptability of such floors is an essential subject in addition to the usual strength criterion. This paper focuses on the dynamic response of a lightweight composite structural system known as Profiled Steel Sheet Dry Board (PSSDB) to evaluate its vibration acceptability under human walking load. For this point, twelve (12) PSSDB panels in the category of Low Frequency Floor (LFF) were developed via Finite Element Method (FEM). The natural frequencies and mode shapes of the studied panels were determined based on the developed finite element models. For more realistic evaluation on dynamic response of the panels, dynamic load models representing human walking load were considered based on their Fundamental Natural Frequency (FNF), and also time and space description. The peak accelerations of the studied panels were determined and then compared to the limiting value proposed by the standard code of ISO 2631-2. Effects of changing thickness of the Profiled Steel Sheet (PSS) and Dry Board (DB), screw spacing, damping ratio, type of support, and floor span on the dynamic responses of the PSSDB panels were evaluated. According to literature, effect of presence of concrete on the dynamic response of the PSSDB system was revealed. The results demonstrated that although some factors reduced dynamic response of the PSSDB system under human walking load, low frequency PSSDB floor system could reach high vibration levels resulting in lack of comfortableness for users.